GEANS releases fully validated laboratory protocol
The described protocol uses organismal DNA and is based on results of two pilot tests conducted by ILVO. Macrobenthos from the Belgian part of the North Sea was morphologically identified prior to molecular processing. The molecular lab protocol includes guidelines to prevent contamination and step-by-step information to conduct blending, DNA extraction, amplification of the COI gene and library preparation for next generation sequencing . The protocol was cross-validated by partners Senckenberg, Naturalis and Aarhus University, and made publicly available at laboratory-protocol-for-dna-metabarcoding-of-soft-sediment-macrobenthos-from-the-north-sea.pdf (northsearegion.eu). Looking forward to citations!